Project

Part:BBa_K3113302:Design

Designed by: Alejandro Salinas Illarena   Group: iGEM19_Munich   (2019-10-12)


pCAG_Gag-HiBiT-L7Ae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3019
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2319


Design Notes

This sequence was optimised to function in mammalian cells.


Source

CAG promotor, Gag: HIV coat protein, HiBit: small part of nanoluc luciferase, L7Ae : archeal ribosomal RBP

References